FIG 11.

mTORC1/2 inhibition partially restores G₁ checkpoint arrest. (A) SUM149 cells were treated with PP242 or DMSO 2 h before 8 Gy of IR or mock treatment; 30 min later nocodazole was added, and cells were harvested at the indicated times and fixed for PI staining to assess DNA content. The diagram outlines the experimental design. (B) SUM149 cells were subjected to a double thymidine block. While still under thymidine treatment, cells were treated with PP242 or DMSO as described above for 2 h before IR at 8 Gy, followed by washout of the thymidine block. Cells were harvested and fixed for PI staining at the indicated time points. The diagram indicates the experimental design.