FIG 4.

Analysis of gene expression in cdKO MEFs. (A) Expression of Tgif1 and Tgif2 was analyzed by qRT-PCR in wild-type and cdKO MEFs after tamoxifen treatment. (B) Expression of a selection of genes (primarily from the overlap between Tgif1 null MEFs and cdKO embryo RNA-seq) was analyzed by qRT-PCR in wild-type and cdKO MEFs. (C) Analysis of cilium-related gene expression by qRT-PCR in wild-type and cdKO MEFs. The relative expression based on embryo RNA-seq is shown at the top for comparison. *, P < 0.05; **, P < 0.01; ***, P < 0.001.