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. 2017 Feb 15;37(5):e00456-16. doi: 10.1128/MCB.00456-16

TABLE 2.

Primers used for Flag-tagged AEG-1 fragment plasmid construction

Primer Sequence
AEG-1-N1-up CGCGGATCCGCCATGTGGGCCGCGGCTTGCGCCGGCTC
AEG-1-N2-up CGCGGATCCGCCATGGACGACCTGGCCTTGCTGAAGAATC
AEG-1-N3-up CGCGGATCCGCCATGCGTAAACGTGATAAGGTGCTGACTG
AEG-1-N4-up CGCGGATCCGCCATGACCGAGCAACTTACAACCGCATC
AEG-1-N5-up CGCGGATCCGCCATGTCTGGAAAAGGAGATTCTACACTTC
AEG-1-N6-up CGCGGATCCGCCATGTCTGTAAAACTCTCCTCACAGATC
AEG-1-N-dn TGCTCTAGATCACTTGTCATCGTCGTCCTTGTAGTCCGTTTCTCGTCTGGCTTTTTTC
AEG-1-N7-up CGCGGATCCGCCATGGACTACAAGGACGACGATGACAAGCAGTCTACCACTTCTGATTA
AEG-1-N7-dn CCGGAATTCTCACGTTTCTCGTCTGGCTTTTTTC
AEG-1-C-up CGCGGATCCGCCATGGCTGCACGGAGCTGGCAGGACGAG
AEG-1-C1-dn TGCTCTAGATCACTTGTCATCGTCGTCCTTGTAGTCAAGAGTCTTGATAGGCTGGC
AEG-1-C2-dn TGCTCTAGATCACTTGTCATCGTCGTCCTTGTAGTCCCAATTGCCCCACTCTTCTG
AEG-1-C3-dn TGCTCTAGATCACTTGTCATCGTCGTCCTTGTAGTCAACTGGCTCAGCAGTAGACC
AEG-1-M1-up CGCGGATCCGCCATGGACTACAAGGACGACGATGACAAGGGAAAAGGAGATTCTACAC
AEG-1-M1-dn CCGGAATTCTCAAACTGGCTCAGCAGTAGACCC
AEG-1-M2-up CGCGGATCCGCCATGGACTACAAGGACGACGATGACAAGGAGCAACTTACAACCGCATC
AEG-1-M2-dn CCGGAATTCTCAAACTGGCTCAGCAGTAGACCC
AEG-1-M3-up CGCGGATCCGCCATGGACTACAAGGACGACGATGACAAGCGTAAACGTGATAAGGTGC
AEG-1-M3-dn CCGGAATTCTCACCAATTGCCCCACTCTTCTGC
AEG-1-M4-up CGCGGATCCGCCATGGACTACAAGGACGACGATGACAAGTGGGCCGCGGCTTGCGCCGG
AEG-1-M4-dn CCGGAATTCTCATGGAAGAGTCTTGATAGGCTGGC