Skip to main content
. 2016 Aug 1;36(6):731–745. doi: 10.1038/onc.2016.242

Figure 5.

Figure 5

miR-372-3p reduces the expression of oncomotif-miRNA targets. (a) RNA sequencing results of several previously described and validated targets of different oncomotif-miRNAs. mRNA levels in miR-372-3p mimic-transfected cells are shown in relation to non-targeting siRNA ctrl-transfected cells. Indicated are also the results from a t-test analysis based on the RNA sequencing data (***P-value<0.001, **P-value<0.01, *P-value<0.05 and NS=non-significant). (b) Quantitative MS results of the same targets. Protein levels in miR-372-3p mimic-transfected cells are shown in relation to non-targeting siRNA ctrl-transfected cells. Indicated are also the results from a t-test analysis based on the MS analysis (P-values are indicated as in (a), NS=non-significant, ND=not determined). (c) Western blot analysis of miR-372-3p mimic effect on selected miR-372-3p targets in four different NSCLC cell lines. GAPDH was used as a loading control, and the figure shows representative blots from three replicate experiments.