FIG 10.

Pictorial depiction of the approach to construct the gfp insertion vector. fhbA and bta003 were amplified using primers (A), adding the appropriate restriction enzyme sites (B), and digested and ligated (C). The ligation reaction was cloned into the PCR2.1 TOPO vector, generating PCR2.1::fhbA-bta003 (D, left plasmid). The kanamycin acetyltransferase gene and gfp driven by the B. turicatae flaB promoter (P_flaB kan-gfp) were amplified by PCR adding AvrII and NheI restriction sites to the 5′ and 3′ ends of the amplicon, respectively, and cloned into PCR2.1::fhbA-bta003 (D, left plasmid, and E).