FIG 3.

Evaluation of B. turicatae-gfp in vitro and in murine blood after needle inoculation. B. turicatae-gfp grown in vitro is shown under fluorescent (A) and dark-field microscopy (B). In the blood, wild-type spirochetes failed to fluoresce (C) yet were detected by dark-field microscopy (D). (E) B. turicatae-gfp remained fluorescent in the blood after needle inoculation. Images were captured using a 63× oil immersion objective, and a scale is shown as a white bar toward the bottom left of each image.