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. 2016 Sep 26;36(6):840–849. doi: 10.1038/onc.2016.252

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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Regulation of the TGFβ–CXCL12–CXCR7 axis in MSCs and tumour cells can be observed in human cells. (a) CXCL12 expression in different tissue-derived human MSCs was analysed by real-time PCR. ADSC, adipose-derived MSC; AMSC, amniotic fluid-derived MSC; BMSC, bone marrow-derived MSC; UCSC, umbilical cord-derived MSC. (b, c) TGFβ inhibits CXCL12 expression in human MSCs. Cultured human ADSCs (b) and AMSCs (c) were stimulated with TGFβ (10 ng/ml) for 12 h. SB431542 (10 μM) was added to block the TGFβ receptor. CXCL12 expression was assayed by real-time PCR. Data were shown as means±s.e.m. (d) The expression of CXCR4, CXCR7 and CXCL12 in cultured MCF7 cells was analysed by real-time PCR. (e) MCF7 cells were treated with ADSC-conditioned medium or recombinant human CXCL12 protein (20 ng/ml) for 2 days. Cells were analysed for CXCR7 expression by flow cytometry.