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. 2017 Jan 5;8(2):432–445. doi: 10.1016/j.stemcr.2016.12.009

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

CtIP Is Essential for Differentiation and Genomic Stability Maintenance in iPSCs

(A) Median size of embryonic bodies (EBs) generated at 6 days after spontaneous differentiation of miPSCs reprogrammed in the presence of shCtIP or control shNT. At least 300 EBs of each condition were measured. The average and SD of five independent experiments is plotted. Statistical significance was obtained by a t test.

(B) Representative images of EBs using BF and GFP filters.

(C) Western blot analysis of indicated proteins in EBs generated from miPSCs reprogrammed in the presence of shCtIP or control shNT. A representative western blot is shown of three independent experiments.

(D) Same as in (A), but with cells reprogrammed in the presence of CtIP and then transduced with shCtIP or shNT as indicated. ns, not significant.

(E) Same as in (C) but with cells transduced with shCtIP or shNT after reprogramming.

(F) Copy number variation (CNV) of genomic DNA between iPSCs obtained in the presence or absence of CtIP during cell reprogramming. Two independent experiments (the first and second assays) are shown. CNVs were detected using SurePrint G3 Unrestricted CGH (4 × 180 K) arrays. See also Figure S5.