Figure 2.

ESC Cultures Contain Different PDGFRα⁺ Subpopulations

(A) Representative intracellular FACS analysis for OCT4, GATA4, and NANOG on the R1 line, n = 3. The gating strategy was based on isotype controls.

(B) qRT-PCR analysis for embryonic and extraembryonic markers in the three subpopulations. Data are presented as means ± SEM of each transcript from three independent experiments (normalized to β-Actin), ^∗p < 0.05, t test.

(C) Representative western blot of three independent experiments for OCT4, NANOG, SOX2, GATA4, and GATA6 on sorted cells. B-TUBULIN was used as normalizer.

(D) Bright field pictures and alkaline phosphatase staining on sorted subpopulations. Scale bar, 100 μm.

(E) Percentage of each subpopulation 1 week after their respective sorting, calculated from three independent experiments, ^∗p < 0.05, t test.

See also Figure S3.