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. 2017 Mar;23(3):355–364. doi: 10.1261/rna.059824.116

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Published by Cold Spring Harbor Laboratory Press for the RNA Society.

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 9. — Chelation of Ca²⁺ by EGTA halts glmS^Ca, but not glmS^WT or glmS^AAA under physiological cation concentrations. Reaction time courses for (A) glmS^WT (in the presence of 25 µM GlcN6P), (B) glmS^AAA, and (C) glmS^Ca were initiated by the addition of both 25 mM Mg²⁺ and 25 mM Ca²⁺. After the second time point, half of the sample from each reaction was transferred into a fresh tube containing a twofold molar excess of EGTA. The reactions with (red) or without EGTA (blue) added were continued for the remaining time points. First-order rate fits are shown for reactions with no EGTA added (blue). Error bars are standard errors of the mean from three independent time courses.