FIGURE 3.

Gcn4 is activated in nup100Δ mutants and required for their increased longevity. (A) Relative levels of β-gal activity from the lysates of strains transformed with GCN4-lacZ (p180) (Hinnebusch 1985). An asterisk indicates a P-value of less than 0.05 when compared to wild type using a two-tailed Student's t-test with an n of at least six. (B–D) Survival curves for the indicated strains; average life spans for each are listed in parentheses next to the strain name. An asterisk indicates a P-value of less than 0.05 when the values were compared to wild type using a two-tailed Mann–Whitney U-test with an n of at least 60 cells per strain. (E) Survival curves for nup100Δ gcn4Δ cells transformed with the listed integrating vectors. An asterisk indicates a P-value of less than 0.05 when the values were compared to the strain transformed with the empty vector using a two-tailed Mann–Whitney U-test with an n of at least 45 cells per strain.