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. 2017 Mar;23(3):406–419. doi: 10.1261/rna.059667.116

FIGURE 5.

FIGURE 5.

t6A37 and i6A37 are important for m3C modification of tRNASer species. (A) t6A37 is important for m3C modification of tRNASer(GCU). Bulk RNA from SUA5+ and sua5Δ cells was analyzed by primer extension with a primer annealing to residue e7–35 of tRNASer(GCU). (B) i6A37 is important for m3C modification of tRNASer(UGA) and tRNASer(CGA). Bulk RNA from MOD5+ and mod5Δ cells was analyzed by primer extension with a primer annealing to residue e8–36 of tRNASer(CGA). (C) i6A37 is important for tRNASer(CGA) binding to ORF240. ORF240-PT was expressed in MOD5+ and mod5Δ strains, and ORF240 tRNA binding was analyzed by the IgG Sepharose pull-down assay as described in Figure 3C. (D) Schematic of tRNASer with highlighting of the V-loop containing residues Ne1–e9 (black) and five other nucleotides (gray).