Fig. 7.

H3K27me3 is associated with native germline nuclear RNAi targets and not affected in met-2 set-25;set-32 mutant worms. a, b Scatter plots comparing whole-genome H3K27me3 levels in WT and hrde-1 or met-2 set-25;set-32 mutants at 1 kb resolution. c Boxplot analysis showing H3K27me3 changes between WT and met-2 set-25;set-32 or hrde-1 mutant worms in different subsets of the genome: GRTS loci (191 kb), GRH loci (215 kb), top 5-percentile H3K27me3 regions in WT (H3K27me3 + regions, 4966 kb). d Boxplot analysis comparing mRNA expression between WT and mes-2, hrde-1, or met-2 set-25;set-32 mutants in different subsets of the genome: GRTS loci (121 genes), GRH loci (142 genes), genes that are up-regulated in mes-2 mutant worms (355 genes, ≥2 fold, FDR < 0.05). Published microarray data [48] were used for mRNA expression changes between mes-2 mutant and WT worms