Table 1.
Bacterial strains used in the present study
| Strain | Phenotype/genotype | Source or reference |
|---|---|---|
| Salmonella | ||
| S. Enteritidis NCTC13349 | Wild-type strain | [13] |
| S. Enteritidis ∆SPI-13 | NCTC13349 ∆(SEN2960-SEN2966)::FRT | This study |
| S. Enteritidis ∆SPI-13/pSPI-13 | NCTC13349 ∆(SEN2960-SEN2966)::FRT/pBAD-TOPO::(SEN2960-SEN2966) | This study |
| S. Typhi STH2370 | Wild-type strain | [26] |
| S. Typhi ∆SPI-8 | STH2370 ∆(STY3273-STY3292)::FRT | This study |
| S. Typhi ∆SPI-8/pSPI-8 | STH2370 ∆(STY3273-STY3292)::FRT/pBAD-TOPO::(STY3273-STY3292) | This study |
| S. Typhi ∆SPI-8/pSPI-13 | STH2370 ∆(STY3273-STY3292)::FRT/pBAD-TOPO::(SEN2960-SEN2966) | This study |
| Escherichia coli | ||
| TOP10 | K12 F− Ф80lacZ∆M15 ∆(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(r −k, m +k) phoA supE44 thi–1 gyrA96 relA1 λ− | Invitrogen |