Table 1.
IC50 value of aqueous and methanol extracts of Piptadeniastrum africanum on human whole blood evaluated by luminol and/or lucigenin amplified chemiluminescence
| Oxidative burst (IC50 μg/ml) | T-cells proliferation (IC50 μg/ml) | Cytotoxicity on 3T3 Cells (IC50 μg/ml) | ||||||
|---|---|---|---|---|---|---|---|---|
| Luminol and Zymosan | Lucigenin and PMA | |||||||
| WB | PMNs | MQ | WB | PMNs | MQ | |||
| Aqueous extract | 10.04 ± 0.31 | 8.68 ± 0.52 | 11.64 ± 0.29 | 8.59 ± 0.12 | 5.74 ± 0.38 | 11.85 ± 0.36 | 29.30 ± 0.36 | 78.60 ± 0.35 |
| Methanol extract | 4.02 ± 0.10 | 2.29 ± 0.10 | 2.85 ± 0.12 | 4.89 ± 0.28 | 2.96 ± 0.07 | 2.80 ± 0.02 | <1 | 6.13 ± 0.25 |
| Ibuprofen | 13.00 ± 0.30 | 12.97 ± 0.23 | 13.93 ± 0.52 | 13.73 ± 0.58 | 13.69 ± 0.61 | 14.30 ± 0.49 | - | - |
| Prednisolone | - | - | - | - | - | - | <3.1 | - |
| Cyclohexamide | - | - | - | - | - | - | - | 0.1 ± 0.13 |
The IC50 values are presented as mean ± SD of triplicates. Where WB whole blood, PMNs polymorphonuclear leukocytes, MQ mice peritoneal macrophages