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. 2017 Feb 16;7:42801. doi: 10.1038/srep42801

Figure 7. Exposure of 3T3-L1 pre-adipocytes to BAR502 increases Ucp1 expression.

Figure 7

(A) 3T3-L1 pre-adipocytes were differentiated into mature white adipocytes for 4 days and then stimulated 24 hours with 10 μM BAR502. (B) Total RNA was extracted from cells and used to evaluated the relative mRNA expression of adipogenic marker genes, genes involved in brite differentiation and autophagic genes by RT-PCR. Results are the mean ± SE of 2 experiments. *p < 0.05 versus undifferentiated cells (Day 0); #p < 0.05 versus differentiated cells (Day 4). Values are normalized to GAPDH, the relative mRNA expression is expressed as 2(−ΔΔCt). (C) Expression of UCP1 wa also investigated by western blot analysis. Then Western blot shows that exposure of 3T3 –L1 cells to BAR502 increases the expression of UCP1 protein. The blot shown is representative of two others showing the same pattern.