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. 2017 Feb 10;6(2):e127. doi: 10.1038/cti.2017.1

Figure 4.

Figure 4

B-LCLs modulate Th17 responses. The effect of B-LCLs on Th17 responses was analyzed by classic Th17-associated markers by FACS and with the ProcartaPlex Luminex assay. Mononuclear cells derived from the axillary lymph nodes (LNs) of marmosets (n=14) immunized with MOG34–56/IFA were co-cultured with CellTrace-labeled B-LCLs with or without the addition of MOG34–56 for 48 h. A transwell system in which B-LCLs and LNs were separated was used as a control (no MOG34–56 was added). Supernatant was collected to measure cytokine production and cells were analyzed by FACS. Shown is the percentage of cells expressing either IL-23R (a) or CCR6 (b) from the parent gate indicated above the graphs. (a and b) Reductions in the percentage of IL-23R- and CCR6-expressing CD4+ and CD8+ T cells were observed in cells that had lost CD27 expression. In both cases, the addition of antigen was not required for reductions, and not limited to effector populations (data not shown). (b) Reductions in CCR6 were observed at initial co-culture periods (0 h) taken for baseline measurements. (c) Stimulation of B-LCL and antigen elicited significantly higher levels of IL-17A and IFN-γ compared to their respective controls following 48 h of co-culture. Secretions of both cytokines were dependent upon the addition of antigenic peptide. Detection of IL-23p19, IL-12p70 and IL-2 was absent after 24 and 48 h (data not shown). Statistical significance of P<0.05 is indicated by *, significance of P<0.01 is indicated by ** and P<0.001 is indicated by ***.