Figure 6. Mitochondrial peripheral retraction is linked to reduction in IMC tight-proximity zones.

(a) Schematic depiction of two tachyzoites showing their mitochondria (green), plasma membrane (grey), microtubules (dark purple), inner membrane complex (magenta) and sub pellicular microtubules (fuzzy grey). (b) Cryo-immuno-EM using parasites expressing the mitochondrial associated TgElp3-HA²² (labeled with gold beads). White arrows show IMC. Black arrows show the plasma membrane. (c) Frequency of sections showing patches of mitochondrial–IMC proximity of <50 nm or >50 nm distance and of section not showing mitochondrial profile, among EM sections of intracellular and extracellular parasites. (d) Snapshots from the time-lapse microscopy shown in Movie S7. Merge panel shows TGME49_ 215430 in green and IMC3 in magenta, and each channel is also shown separately (IMC in the middle and TGME49_ 215430 at the bottom). Square parentheses highlight regions of close IMC-mitochondrion contact that are stable over the period of live imaging. Arrowheads mark region of transient extensions from the mitochondrial tubule.