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. 2017 Feb 16;7:42704. doi: 10.1038/srep42704

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Copyright © 2017, The Author(s)

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Immunoblot analysis of bacterial pellets (P) and culture filtrates (S) of WT PPE68_1_HA and its variants L125E and L125A in the WT M. marinum, detected with an HA antibody. Intracellular GroEL2 and supernatant protein EsxA were detected as controls. Equivalent OD units were loaded; 0.2 OD for pellet and 0.5 OD for supernatant fractions.