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. 2017 Feb 16;7:42704. doi: 10.1038/srep42704

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Immunoblot analysis visualizing the secretion of different PE35/PPE68_1 variants containing an HA-tag at the C-terminus of PPE68_1, using cell pellets (P) and culture supernatant (S) of WT M. marinum (A), eccCb₁ mutant (B) and ∆espG₁ (C) strains. GroEL2 and EsxA were used as loading controls. Equivalent OD units were loaded; 0.2 OD for pellet and 0.5 OD for supernatant fractions.