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. 2017 Feb 16;7:42704. doi: 10.1038/srep42704

Figure 5. The rerouting of PE35/PPE68_1 SWAP to the ESX-5 system affects the secretion of endogenous ESX-5 substrates.

Figure 5

Surface localization of the ESX-5 dependent PE_PGRS substrates in the WT M. marinum (A), ∆espG1 strain (B) and the eccCb1 mutant strain (C) carrying various PE35/PPE68_1 variants was analyzed by immunoblotting. Bacterial cell pellets (P) were treated with Genapol X-080 to enrich for capsular proteins (S). Equivalent OD units were loaded; 0.2 OD for pellet and 0.5 OD for Genapol X-080 treated surface fractions. GroEL2 was included as loading control.