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. 2017 Feb 16;7:42855. doi: 10.1038/srep42855

Figure 4. Migration rates of cryogel-housed modified MSCs towards stimuli.

Figure 4

(a) Extracellular (i; iii) and intracellular (ii; iv) expression of CXCR4 and TNFR1 receptors on modified MSCs detected via flow cytometry upon staining with anti-CXCR4/PE and anti-TNFR1/PE mAbs (black), or matched isotype control Abs (grey). Fractions and the mean fluorescence intensity (MFI) of total cells are reported. (b) Induced and spontaneous migration (medium without chemoattractants) were examined either in 2D cell cultures or in cryogel-housed MSCs samples (3D) functionalized with 40 μg/ml, 80 μg/ml or 160 μg/ml RGD-containing peptides. Representative images are reported for MSCs migration rates in the presence or absence of chemoattractants for 2D and 3D cell cultures respectively. (c) Percentages of migrated cells are reported as the means ± SD of total cells detected in five random microscope-viewing fields for three independent experiments. Statistical significance was determined using one-way ANOVA with Bonferroni multiple comparison test. **p < 0.01.