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. 2017 Feb 16;7:42855. doi: 10.1038/srep42855

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Copyright © 2017, The Author(s)

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(a) Representative illustration of the cryogel-housed scBsAb-releasing MSCs system. Genetically modified MSCs were confined in the starPEG-heparin cryogels for a constant release of the bsAb CD33-CD3 suitable for the redirection of T-cells to AML target cells over prolonged time. (b) The amount of bsAb CD33-CD3 produced by modified MSCs seeded at reported seeding densities in the cryogel scaffold was quantified by ELISA. The antibody concentration (reported in μg) released in the cell culture supernatants was determined at different cultivation time points for an overall time of 300 h. The cumulative release of the bsAb detected over time is reported as the means ± SD of three independent experiments.