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. 2017 Feb 16;7:42688. doi: 10.1038/srep42688

Figure 1. In vitro polarisation and migratory potential of macrophages.

Figure 1

Stimulation of WT and TLR5−/− MCSF-derived macrophages with Flagellin or LPS did not induce significant differences in iNOS (A) and Arg-1 (B) gene expression between both groups. Already at baseline, WT macrophages showed higher mRNA levels of MCP-1 (C), CCR2 (D) and IL6 (E), of which the expression of MCP-1 and IL6 increased upon Flagellin stimulation. No such increase was observed in TLR5−/− macrophages upon stimulation with Flagellin. The expression of CCR2 was also different at baseline between both groups, but no change in expression level was observed after stimulation with Flagellin (D); n = 4 per group. WT: wildtype, TLR5−/−: toll-like receptor 5 knockout, #p < 0.05, ##p < 0.01, ###p < 0.001, **p < 0.01, ***p < 0.001.