Figure 2. Ang II presented as an autocrine product of tumor cells in hypoxic regions.

(a) The angiotensinogen (AGT) gene was stably silenced in CNE2 tumor cells, as demonstrated by Western blot. (b) AGT-silence considerably reduced Ang II levels in the supernatant of CNE2 and 5–8F tumor cells as detected by ELISA. (c) AGT-silence attenuated Ang II levels in pimonidazole-positive hypoxic regions of in vivo tumors formed by CNE2 cells. *P < 0.05, **P < 0.01.