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. 2017 Feb 16;7:42529. doi: 10.1038/srep42529

Figure 5. Competition results for MccPDI gene knockout and complementation experiments.

Figure 5

(A) CFU counts for E. coli BW25113 following competition with microcin-producing E. coli-25 and associated gene knockout and complemented strains. Results are expressed as the difference in CFUs of the sensitive strain grown in co-culture and monoculture (error bars = SEM; 3 independent experiments). *Significant ANOVA followed by a Dunnett’s one-way multiple comparisons test versus control group (E25) (P < 0.01). (B) Western blot analysis of McpM in different E. coli strains, including BW25113, 186, and mcpM-complementation strains. Samples are from whole cell lysate and endogenous DnaK served as a loading control. Three bands, indicated by black arrows, correspond to the putative full length McpM (top) followed by two cleaved forms of the protein.