Figure 8. Cysteine residues 57 and 90 are required for McpM function.

(A) Western blot analysis of McpM for strains (E25∆mcpM∆mcpA) with replacement of different cysteine residues protein [designated (p207::mcpM XposY) in Table S1 where “X” is the wild-type amino acid, “pos” is the amino acid position, and “Y” is the replacement amino acid]. Endogenous DnaK served as a loading control. (B) Competition assays between the 4 cysteine-residue mutants and BW25113 (vector control). Results are expressed as the difference in CFU of the sensitive strain grown in co-culture and monoculture (bars = SEM; 3 independent replicates). *Statistically significant ANOVA (P < 0.01 with Dunnett’s upper one-sided multiple-comparison test with control).