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. 2017 Feb 16;7:42213. doi: 10.1038/srep42213

Figure 2. Genomic structure of the C. semilaevis dmrt1 gene, design of dmrt1-TALENs and mutated sequences induced by TALENs.

Figure 2

(A) dmrt1-TALENs designed to target exon 1 of the gene. (B) Detection of mutations in the injected embryos by T7E I digestion. (C) Mutant dmrt1 sequences from TALEN injected embryos. The T7E I cut bands were recovered after gel electrophoresis and cloned for sequence analysis. “−” represents deletion of bp, “+” represents addition of bp. The numbers at the right side indicate the number of deleted or inserted base pairs.