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. 2017 Feb 16;7:42213. doi: 10.1038/srep42213

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(A) Phenotype of gonads in the intersex. (a) Testis shaped up-side gonad of the intersex; (b) ovary shaped down-side mutant gonad of the intersex; (c) testes of a pseudomale. (B) Histology of the gonads. (a) Testis of the intersex showing normal male structures; (b) dmrt1 mutant gonad (ovary) of the intersex; (c) testis of a pseudomale. Scale bar, 100 μm. (C) Determination of the genetic sex of the intersex by SSR PCR yielding different sized products for the Z (169 bp) and W (134 bp) chromosomes. (D) Sequences of wild-type and mutated target sites of dmrt1 retrieved from ovary, partial; and only wild-type dmrt1 in testis, partial. (E) Relative mRNA expression of dmrt1, foxl2 and cyp19a1a in the dmrt1-deficient gonad at one year of age. β-actin was used as internal standard.