Figure 3. GABAergic neurogenesis is impaired in Wnt5a cKOs.

(A–F) Immunohistochemical analysis of GABAergic neuronal markers Lhx1/5 (A,B), Mash1 (C,D) and Pax2 (E,F) at E14.5 stage in Wnt5a^f/f and Nestin-cre;Wnt5a^f/f embryos. (G–I) Quantification of Lhx1/5^+ve cells (G), Mash1^+ve cells (H) and Pax2^+ve cells (I) at E14.5. (J–M) Immunohistochemical analysis with Purkinje cell marker Lhx1/5 at PN14 stage. (N) Quantification of Lhx1/5^+ve cells at PN14. (O–Z) Immunohistochemical analysis of Pax2^+ve cells at PN1 (O–R) PN7 (S–V) and PN14 stages (W–Z). (Aa) Quantification of Pax2^+ve cells at PN1, PN7 and PN14. (Ab–Ac) Immunohistochemical analysis of Parvalbumin^+ve cells in the molecular layer at PN14. (Ad) Graph shows the quantification of parvalbumin^+ve molecular layer interneurons. Data expressed as Mean ± SD, n = 3. Scale bar = 50 μm (A–F), 25 μm (O–Z & Ab–Ac)