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. 2017 Feb 16;7:42523. doi: 10.1038/srep42523

Figure 7. Wnt5a regulates proliferation of VZ and RL progenitors in discrete manner during embryonic and postnatal development.

Figure 7

(A–D) Immunostaining with proliferation marker Ki67 at E14.5. (E) Graph shows the quantification of Ki67+ve cells at E14.5. (F–I) Image shows Ki67 staining at PN1 stage in Wnt5a cKO and control. (J) Graph shows the quantification of Ki67+ve cells at PN1. (K–N) Immunostaining with PH3 at PN1. (O) Schematic indicates the area represented in the image (K–N). (P) Graph indicates quantification of PH3+ve cells. (Q–T) Image shows Ki67 staining at PN7 stage in Wnt5a cKO and control. (U) Schematic indicates the area represented in the image (Q–T). (V) Graph indicates quantification of Ki67+ve cells. (W–X) Immunohistochemical analysis with Cyclin D1 in sagittal sections (PN1) of Wnt5af/f control and Nestin-Cre; wnt5af/f cKO (w’ & x’) represents specific magnified area of W and X. (Y) Graph indicates the quantification of Cyclin D1+ve cells at PN1. (Z–Aa) Immunostaining with Ki67 in E18.5 Wnt5a+/+ control and Wnt5a−/− null mutants. (Ab) Graph indicates quantification of Ki67+ve cells both in PWM and EGL of Wnt5a+/+ and Wnt5a−/− null mutants. Image (F–I) and (W–X) were generated by stitching together multiple images using Photoshop software. Data expressed as Mean ± SD, n = 3. Scale Bar = 25 μm (A–D,F–I,K–N & Q–T), 50 μm (W–X & Z–Aa).