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. 2017 Feb 16;7:42523. doi: 10.1038/srep42523

Figure 9. Wnt5a non-canonically regulates genes associated with cerebellar progenitor proliferation.

Figure 9

(A) Schematic of TOPFlash and FOPFlash luciferase constructs. (B) Schematic represents the protocol followed for dual luciferase assay in primary cerebellar culture. (C) Dual luciferase assay with TOPFlash and FOPFlash luciferase reporter constructs. (D) Schematic shows the experimental procedure followed for gene expression analysis using real time PCR. (E) Real time PCR analysis of Cyclin D1, Sox2, Hes1 and Hes5 expression upon treatment with rWnt5a protein and non-canonical Wnt signaling blocker fumagillin. (F) Real time PCR analysis of cyclinD1 and Hes1 upon rWnt5a, DAPT and fumagillin treatment. (G) Schematic showing the pathway for activation of cyclin D1 by Wnt5a. (H) Schematic showing effect of Wnt5a loss during embryonic and postnatal stages on development of cerebellum. Data expressed as Mean ± SD, n = 3.