Figure 3. TLR2 and TLR4 mediate the recognition of CR-LAAO and modulate IL-6 and IL-1β production.

Peritoneal macrophages from C57BL/6 mice (wild type, TLR2^−/− and TLR4^−/− knockouts) were stimulated with CR-LAAO (0.19, 0.38, 0.75 or 1.5 μg/mL) for 24 h in a 5% CO₂ atmosphere at 37 °C. The supernatants were collected and the levels of IL-6 (A,C) and IL-1β (B,D) were measured by ELISA. Peritoneal macrophages without stimulation were used as negative controls (NC). Data were expressed as means ± SD (n = 6). *p < 0.05 compared to NC.