Figure 4. CR-LAAO induces IL-6 and IL-1β production in tumor cells.

HL-60 and HepG2 tumor cells (2 × 10⁵ cells) were stimulated with CR-LAAO (0.75, 1.5; 1.7 or 10.78 μg/mL) for 6 h in a 5% CO₂ atmosphere at 37 °C. The supernatants were collected and the levels of IL-6 (A,C) and IL-1β (B,D) were measured by ELISA. Tumor cells without stimulation were used as negative controls (NC). Data were expressed as means ± SD (n = 6). *p < 0.05 compared to NC.