Figure 7. The expression and phosphorylation of STAT6 in IL-4/IL-13-treated Lyn^+/+ 16HBE cells (Lyn^+/+ for Lyn-expression lentiviral transfectd cells, control for control vector transfected cells).

The 16HBE cells were infected with the Lyn-expression lentiviral vector and control vector respectively (10⁹ viral particles per well of a 6-well plate). (A) Western blot analysis of Lyn, STAT6 and phosphorylated STAT6 in Lyn^+/+ and control cells after exposure to IL-4. (B) Relative density of STAT6 and phosphorylated STAT6 in IL-4-treated Lyn^+/+ and control cells. (C) Western blot analysis of Lyn, STAT6 and phosphorylated STAT6 in Lyn^+/+ and control cells after exposure to IL-13. (D) Relative density of STAT6 and phosphorylated STAT6 in IL-13-treated Lyn^+/+ and control cells. β-actin was used as the loading control. All data are representative of three experiments, and a statistical analysis was performed.