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. 2017 Feb 1;10(2):127–139. doi: 10.1242/dmm.027185

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — Analysis of DA metabolic enzymes in snail brain homogenates. (A) Western blot with anti-COMT. The bottom panel shows detection of anti-β-actin as a loading control. The top panel shows S- and MB-COMT localization at ∼23 and 26 kDa. A non-linear adjustment (+0.5% level adjustment) was applied to the MB-COMT band in rotenone group. Densitometry evaluations of S-COMT (B) and MB-COMT (C) (n=3, calculated from independent WB experiments). S-COMT: F₍₂₎=135.80, P<0.001; post hoc test between control and rotenone groups (P<0.001), control and rotenone+PACAP groups (P<0.001). MB-COMT: F₍₂₎=50.68, P<0.001; post hoc test between control and rotenone (P<0.001), rotenone and rotenone+PACAP groups (P<0.001). ***P<0.001 between control and treated groups; ^###P<0.001 between rotenone and rotenone+PACAP groups.