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. 2017 Feb 15;144(4):590–600. doi: 10.1242/dev.139956

Fig. 1.

Fig. 1.

Rspo1 is required for HSC specification. The HSC markers runx1 (A,B,D,E) and cmyb (G,H,J,K), which identify HSCs emerging from the dorsal aorta, as well as the T lymphocyte marker rag1 (M,N,P,Q), found in thymic T cells, were examined at 24 hpf, 36 hpf and 96 hpf as indicated, by whole-mount in situ hybridization (WISH) in wild-type (WT; A,G,M), Rspo1 MO-injected (B,H,N), Rspo1 wild-type and heterozygous siblings (D,J,P), or rspo1 dtty135 homozygous mutants (E,K,Q). Red arrowheads indicate HSC precursors in the dorsal aorta (A,B,D,E,G,J,K) or T lymphocytes in the thymus (M,P), when present. Numbers of embryos displaying the depicted phenotype are indicated. (C,F,I,L,O,R) Histograms illustrate percentages of phenotypes. Lateral mid-trunk or ventral head views at 160× magnification.