Fig. 3.

Trk, PTTH and Tsl stimulate Tor activation of AP1- and hkb enhancer-driven Fluc activity and MAPK phosphorylation. (A,C) S2R+ cells were co-transfected with the R-luc transfection control plasmid and an AP-1-dependent Fluc reporter (A) or an hkb enhancer-driven Fluc reporter (C). The experimental plasmid(s) that each set of transfected cells received is shown below each bar. Data represent average±s.d. of three readings, repeated seven times (n=7). Statistical significance was determined with respect to relative Fluc activity in cells expressing Tor alone. ***P<0.001. (B) Western blot analysis of extracts from S2R+ cells transfected with plasmids expressing the proteins shown at the top of each lane. Homogenates were divided in half and run on duplicate gels that were blotted and probed with either anti-phospho-p44/42 MAPK/pERK (top panels) or anti-α-Tubulin as a loading control (bottom panels).