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. 2017 Feb 15;144(4):677–686. doi: 10.1242/dev.146076

Fig. 5.

Fig. 5.

Co-expression with Trk or Tsl leads to dimerization of Tor. (A-D″) Fusion proteins between Tor and the Venus protein N-terminus (VN) or C-terminus (VC) were co-expressed either alone or with Trk or Tsl. The appearance of Venus fluorescence indicates that dimerization of Tor receptors has occurred. The left column shows Venus fluorescence, the middle column shows bright-field images and the right column displays an overlay of the two. (A-A″) Co-expression of Tor[4021]-VN and Tor[4021]-VC. (B-D″) Co-expression of wild-type Tor-VN and Tor-VC in the absence of other introduced genes (B-B″), with Trk (C-C″) or with Tsl (D-D″). Scale bar: 10 μm. (E) Quantitation of the percentage of cells that exhibited bimolecular fluorescence complementation (BiFC) (see also Fig. 6). Data are mean±s.d. derived from three independent replicate experiments (n=3). The total number of cells counted is shown above each bar. ***P≤0.001.