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Exogenously added Trk or Tsl induces dimerization of Tor. (A-D″) Cells co-expressing wild-type Tor-VN/Tor-VC were exposed to CM from cells transfected with vector alone (Vec) (A-A″) or cells expressing Trk (B-B″), Tsl (C-C″) or both Trk and Tsl (D-D″). The left column shows Venus fluorescence, the middle column shows a bright-field image and the right column displays an overlay of the two. Scale bar: 10 μm. (See Fig. 5E for quantitation.)
