Fig. 8.

Trk and Tsl synergize to activate Tor-dependent STAT92E-driven Fluc in cells expressing low levels of Tor. (A-C) S2R+ cells were co-transfected with STAT92E-Fluc/R-luc constructs and the level of STAT92E-directed Fluc expression was assessed. (A) S2R+ cells were additionally transfected with increasing amounts of plasmid DNA carrying Tor (red), Tor plus Trk (yellow), Tor plus Tsl (green) or Tor plus both Trk and Tsl (blue). (B) S2R+ cells were transfected with 5 ng of the Tor expression plasmid plus a concentration range of plasmids bearing Trk alone (yellow), Tsl alone (green) or Trk plus Tsl (blue). (C) S2R+ cells in all samples were transfected with 5 ng of the Tor expression plasmid and exposed to control CM from cells transfected with vector alone (red), CM from cells expressing Trk (yellow) or Tsl (green), or a 1:1 mixture of Trk and Tsl CM (blue). CM was either untreated (1×), or was concentrated by various amounts (2× to 10×). Data are mean±s.d. of three readings, repeated five times (n=5). Statistical significance was calculated with respect to relative Fluc activity for cells expressing Tor alone at the appropriate experimental concentration (0.5-200 ng/well) (A), Tor alone at 5 ng/well (B), or Tor alone (5 ng/well) plus control vector CM at the correct experimental concentration (1-10X concentrated) (C). (A) ***P=0.0010, *P=0.036. (B) *P=0.0202, ***P=0.0082. (C) **P=0.0086, *P=0.0421. ns, not significant.