FIG 4 .

Binding of purified hJAM1-Fc protein to Hom-1 VP1 and the VP1 P domain by LAPD. Lysates of CHO cells expressing Hom-1 VP1 and the VP1 P domain fused to Renilla luciferase (Ruc) were prepared at 72 h posttransfection with the corresponding expression vectors as described in Materials and Methods. CHO cells expressing Ruc alone served as a negative control. Equal amounts of RLU were incubated with recombinant hJAM1-Fc or with control hFc protein. The complexes formed were pulled down with protein A/G beads, and following washing, luciferase activity was measured with the coelenterazine substrate. Data represent the mean and standard error of RLU determined from two replicate experiments. CMV, cytomegalovirus.