FIG 8 .

Anti-hJAM1 antibodies reduce Hom-1 infection. CHO, CHO-T, CHO-J, and CHO-hJAM1-GFP cells (n = 10⁶) were pretreated with PBS, isotypic control MAbs, or BV16, a MAb specific for hJAM1. After pretreatment, Hom-1 was added to the cells at an MOI of 0.1 and cells were incubated for 1 h before replacement of virus-BV16 inocula with growth medium. At 15 hpi, the infected cells and growth medium were collected. The collected samples were subjected twice to a freeze-thawing procedure and virus titers in Vero cells were measured with plaque-forming assay.