FIG 1 .

PcydAB induction identifies DG70 as a candidate respiratory inhibitor. (A) The relative induction of the PcydAB::mWasabi reporter as measured by determining the relative fluorescence units of each drug-treated culture compared to the DMSO-only control is shown for several anti-TB drugs as well as DG70. Each was tested in triplicate in a 96-well format. Isoniazid (INH), rifampin (RIF), and ethambutol (EMB) were used as negative controls; thioridazine (THZ), bedaquiline (BDQ), and PA824 were used as positive controls (21–23). Means and standard deviations from three independent experiments are shown. DG70 consistently induced Pcyd 1.7-fold over DMSO. The Student t test was used to determine statistical significance between DMSO and DG70. *, P ≤ 0.05. (B) Molecular structure of DG70.