TABLE 1 .
DG70 MICs in drug-susceptible, drug-resistant, and menG-overexpressing strains of M. tuberculosis and BCGa
| Strain and supplement | Drug resistance | Strain type | MIC90 (µg/ml) of drug: |
|
|---|---|---|---|---|
| DG70 | INH | |||
| M. tuberculosis | ||||
| H37Rv | No | Lab | 4.8 | 0.05 |
| H37Rv + 400 µM MK4 | No | Lab | 9.6 | 0.05 |
| 210 | No | Clinical | 4.8 | 0.05 |
| 692 | No | Clinical | 1.2 | 0.05 |
| TDR 31 | INH, RIF, EMB, KAN, SM, CAP | Clinical | 1.2 | >3.15 |
| TDR 116 | INH, EMB, PAS | Clinical | 9.6 | >3.15 |
| TDR 36 | INH, RIF, EMB | Clinical | 3.13 | >3.15 |
| TDR 91 | RIF, EMB, INH | Clinical | 4.8 | >3.15 |
| BDQr H37Rv | BDQ | Clinical | 4.8 | 0.05 |
| PA824r H37Rv | PA824 | Lab | 4.8 | 0.05 |
| M. bovis | ||||
| BCG | No | Lab | 2.4 | 0.05 |
| BCG + 300 µM MK4 | ND | Lab | 9.8 | ND |
| BCG::pMV306PHsp60 | HYG | Lab | 2.4 | ND |
| BCG::pMV306PHsp60-menG_Wt | HYG | Lab | 9.8 | ND |
| BCG::pMV306PHsp60-menG_F118L | HYG | Lab | >40 | ND |
| BCG::pMV306PHsp60-menG_V20A | HYG | Lab | 20 | ND |
a
Abbreviations: EMB, ethambutol; RIF, rifampin; INH, isoniazid; KAN, kanamycin; SM, streptomycin; CAP, capreomycin; PAS, para-aminosalicylic acid; BDQ, bedaquiline; HYG, hygromycin B; ND, not determined. MIC90 values were determined by microtiter alamarBlue assay. All MIC90 assays were performed in triplicates.