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. 2017 Feb 14;8(1):e02082-16. doi: 10.1128/mBio.02082-16

FIG 1 .

FIG 1 

PmtA deforms model membranes in vitro. (A) Phospholipid composition of A. tumefaciens membranes (25). (B) Liposome cosedimentation assay with liposomes containing lipids of different A. tumefaciens strains. Recombinant PmtA (10 µM) was incubated with ~0.5 mg/ml liposomes. Supernatant (S) and pellet (P) fractions were analyzed by SDS-PAGE and Western blotting. PmtA was detected with an anti-His antibody. wt, wild type. (C) Membrane-remodeling activity of PmtA assayed via the liposome-remodeling assay. Liposomes (~0.5 mg/ml) containing the indicated lipids from different A. tumefaciens strains were incubated with 10 µM recombinant PmtA (+ PmtA). Samples were incubated for 30 min at room temperature. Liposomes were negatively stained using 1% uranyl acetate and imaged with a Philips 420 transmission microscope. Data are representative of three independent experiments. Bars represent 100 nm. Abbreviations: PE, phosphatidylethanolamine; MMPE, monomethyl-phosphatidylethanolamine; DMPE, dimethyl-phosphatidylethanolamine; PC, phosphatidylcholine; PG, phosphatidylglycerol; CL, cardiolipin.