FIG 3 .

Membrane-remodeling activity of different PmtA variants. (A, top) Schematic representation of PmtA with highlighted membrane-binding regions αA and αF. (Bottom) Helical wheel illustration of αA (amino acids 6 to 24) generated via HELIQUEST (38). The mean hydrophobicity (<H>) and the hydrophobic moment (<µH>) are indicated in the figure. Hydrophobic residues (yellow), basic residues (blue), acidic residues (red), glutamine (rose), and uncharged residues (gray) are indicated. (B and C) Liposome-remodeling assay with truncated PmtA variant (ΔαA) (B) and different point mutants (K6Q R8Q K12Q, F19A, and F13A V24A) (C) with model membranes containing a lipid mixture of PE, PG, and CL (7:2:1) compared to wild-type (wt) PmtA. The experimental procedure was performed as described in the legend to Fig. 1. Data are representative of three independent experiments. Bars represent 100 nm. Asterisks mark exchanged residues.