FIG 7 .

Requirements for liposome-shaping activity of PmtA. (A) The dual liposome-remodeling capacity of PmtA is dependent on the membrane lipid composition. Membrane tubulation is promoted by the presence of cardiolipin (CL), whereas vesiculation of membranes requires CL and monomethyl-phosphatidylethanolamine (MMPE). Proteins, membrane tubules, and vesicles are not drawn to scale. (B) The N-terminal lipid-binding helix αA is important for membrane deformation by PmtA. αA is a multifunctional domain involved in membrane adsorption by the membrane-binding motif (MBM) comprised of R8, K12, and F19 (23) and membrane shaping by the predicted hydrophobic face.