FIG 4 .

FANCD2 colocalizes with components of the ATM pathway in discrete nuclear foci. (A) HFKs and CIN612 cells were differentiated for 72 h in 1.5 mM calcium medium. Western blot analysis was performed using antibodies to FANCD2, FANCI, BRCA1, BRCA2, RAD51, and γH2AX. GAPDH was used as a loading control. (B and C) CIN612 cells were differentiated for 72 h in 1.5 mM calcium medium and stained with anti-FANCD2 (green) and either anti-BRCA1, anti-γH2AX, or anti-p-SMC1 (red). Cells were counterstained with DAPI (blue). UD, undifferentiated; D, differentiated.