FIG 7 .

FANCD2 localizes to HPV replication centers. (A) CIN612 cells were differentiated for 72 h in 1.5 mM calcium medium. Immunofluorescence analysis for FANCD2 (red) was performed followed by fluorescent in situ hybridization (I-FISH) for HPV31 DNA (green). Cells were counterstained with DAPI (blue). In undifferentiated cells, the FANCD2 signal overlapped with 42.47% ± 12.17% of the HPV31 DNA signal and 11.55%± 1.479% in differentiated cells. UD, undifferentiated; D, differentiated. (B) CIN612 cells were differentiated in 1.5 mM calcium medium, and immunofluorescence analysis for p-SMC1 (red) was performed followed by fluorescent in situ hybridization for HPV31 DNA (green). Cells were counterstained with DAPI (blue). In differentiated cells, the p-SMC1 overlapped with 31.85% ± 8.54% of the HPV31 DNA signal. The percentage of overlap between the HPV31 DNA signal and either FANCD2 or p-SMC1 was measured using ImageJ area analysis and found to be statistically significant where P is <0.05.